Localization of NORs in chromosomes of mouse cell lines by a combined 33258-Hoechst and Ag-staining technique
نویسندگان
چکیده
منابع مشابه
Short communication: Chromosome description and localization of Nucleolus Organizing Regions (NORs) by Ag-staining Technique in Alburnus filippii (Cyprinidae, Cypriniformes) in Anzali Lagoon, North of Iran
متن کامل
Short communication: Chromosome description and localization of Nucleolus Organizing Regions (NORs) by Ag-staining Technique in Alburnus filippii (Cyprinidae, Cypriniformes) in Anzali Lagoon, North of Iran
متن کامل
Mouse-human heterokaryon analysis with a 33258 Hoechst-Giemsa technique
The bibenzimidazol derivative 33258 Hoechst can be used to distinguish microfluorometrically between mouse and human nuclei in heterokaryons. This affords a quick and accurate alternative to autoradiography in the analysis of such heterokaryons. The 33258 Hoechst fluorescence patterns can be converted after irradiation to a Giemsa rendition of the differential staining.
متن کاملChromosomal Variation in Three Human-Mouse Hybridoma Cell Lines after Various Passaging Intervals as Assessed with Two Different Staining Methods
Objective(s) The main objective of this study was to investigate the status of chromosome stability in 3 human-mouse hybridoma cell lines over a period of time in various passages. Materials and Methods Metaphase spreads from 3 human-mouse cell lines (HF2X653, SPMO-4 and F3B6) that had been cultured in 4 successive passages, from 1 to 4 weeks, were prepared and analyzed. Metaphase chromosome...
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ژورنال
عنوان ژورنال: Hereditas
سال: 2009
ISSN: 0018-0661
DOI: 10.1111/j.1601-5223.1979.tb01291.x